Downregulation of the Gene Expression of Efflux Pump Gene (acrA) and its Regulator (ramA) in Klebsiella pneumoniae by Quaternary Ammonium Compounds

Introduction: Biocides are commonly used for disinfection in a variety of contexts. They generally to avoid infection by controlling biofilm on medical equipment. However, the literature lacks information effect biocide efflux pump gene expression. Objective: To determine influence development and acrA ramA Methodology: The microtiter plate method was identify 80 isolates K. pneumoniae. minimal inhibitory concentrations (MIC) three biocides (quaternary ammonium compound (QAC), chlorohexidine digluconate, chloroxylenol) were estimated. QAC intensity viability biofilms investigated as well. Its evaluated using scanning electron microscopy. For each isolate, expression before after treatment with (at ½MIC). Results: study revealed that 60% formed moderate biofilms. Biofilms all inhibited at high concentrations. Moreover, ½MIC downregulated (acrA) its regulator (ramA). Conclusion: prevented doses. Furthermore, decreased How cite this article:Odaa FH, Al-Mathkhury HJF. Downregulation Gene Expression Efflux Pump Regulator (ramA) Klebsiella pneumoniae Quaternary Ammonium Compounds. J Commun Dis. 2023;55(2):18-27. DOI: https://doi.org/10.24321/0019.5138.202320